皮尔斯/皮尔斯定量比色肽分析/500次分析/23275
商品编号：
23275
品牌：
piercechemical
市场价：
¥6180.00
美元价：
3708.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
TheThermoScientific™Pierce™QuantitativeColorimetricPeptideAssayisaneasy-to-usecolorimetricmicroplateassaydesignedspecificallytoimprovethesensitivityandreproducibilityofquantitationofpeptidemixtures.•Sensitive—accuratelydetectaslittleas25µg/mLofpeptidemixture
•Robust—assayperformancerigorouslytestedusingpeptidedigestmixtures
•Robustpeptidedigeststandard—kitincludesavalidatedpeptidedigeststandardforimprovedreproducibilityofquantitation
•CompatIBLe—workswithmanyreagents,includingmassspectrometrysamplepreparationreagentsandTMT™-labeledpeptides
•Convenient—easymix-andread-formatandstablecolorimetricsignalthatmaybereadinaslittleas15minutes
ThePierceQuantitativeColorimetricPeptideAssayprovidesmodifiedBCAreagentsforthereductionofCu+2toCu+1andaproprietarychelatorthathavebeenoptimizedforthequantitationofpeptidemixtures.Inthisreaction,thecopperisfirstreducedbytheamidebackboneofpeptidesunderalkalineconditions(Biuretreaction).Theproprietarychelatorthencoupleswiththereducedcoppertoformabrightredcomplexwithabsorbanceof480nm.Thesignalproducedfromthisreactionis3-4foldmoresensitivethantheMicro-BCAProteinAssayforpeptideanalysis.
Thekitalsocontainsahighqualitypeptidedigestreferencestandardforuseingeneratinglinearstandardcurvesandtoserveasacalibrationcontrol.Thiscolorimetricpeptideassayrequiresasmallamountofsample(20µL)andhasaworkingpeptideconcentrationrangeof25–1000μg/mL.Theincreasedsensitivity,lowsampleassayvolume,andincludedreferencestandardareessentialforaccurateandrobustmeasurementofpeptidedigestsamples,especiallyformassspectrometryapplications.
Applications:
•Recommendedforpeptidemixtures
•Notrecommendedforsyntheticpeptidesbecausetheassayisaffectedbypeptideaminoacidcontent
•Normalizingsampleconcentrationsforquantitativedownstreamapplications
•NormalizingsampleloADIngvolumesforLC,MS,andLC/MSapplications
•Measuringrecoveryafterpeptideclean-upprocedures
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
SensitivityofPierceQuantitativeFluorometricPeptideAssay
ImprovedSensitivityofPierceQuantitativeColorimetricPeptideAssay
Colorimetricassaychemicalcompatibility
Quantitationcomparisonbetweencolorimetricandfluorometricpeptideassays
Specifications
| DetectionMethod: | Colorimetric |
|---|---|
| FinalProduct: | Peptide |
| ForUseWith(Equipment): | MicroplateReader |
| ProductLine: | Pierce™ |
| ProductSize: | 500assays |
| StartingMaterial: | Peptides, Protease-digestedprotein |
| WorkflowStep: | In-ProcessTesting |
Contents&storage
ColorimettricAssayReagentA,50mL
ColorimetricAssayReagentB,2x25mL
ColorimetricAssayReagentC,2mL
PeptideDigestAssayStandard,1.5mL
Storeinrefrigerator(2–8°C).
ColorimetricAssayReagentB,2x25mL
ColorimetricAssayReagentC,2mL
PeptideDigestAssayStandard,1.5mL
Storeinrefrigerator(2–8°C).
品牌介绍
Pierce创立于1950年，现为生命科学和分析研究领域的领先者。在有机合成、化学偶联、表面化学、分析化学等方面具有强大实力，特色产品是优良的载体蛋白，交联试剂，蛋白及抗体的固化，非放射性标记，蛋白质分析及表达蛋白抽提试剂盒等，为单抗制备、纯化、修饰和蛋白质研究等方面的提供全新的解决方案，其化学发光技术也居于世界领先地位。
