Pierce/Dynabeads M-280 Tosylactivated/2 mL/14203
商品编号：
14203
品牌：
piercechemical
市场价：
¥7120.00
美元价：
4272.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
Whenyouwishtocovalentlycoupleantibodies,peptides,intactproteinsandfunctionalenzymestothesurfaceofamagneticbeadchooseDynabeads®Tosylactivated.Optimalantibodyorientationmakesthesebeadsanexcellentchoicefortheimmunoprecipitationofproteinsandproteincomplexes.IdealforImmunoprecipitationofproteincomplexes:
LowbackgroundandcovalentlinkingofantibodiestothebeadsurfacemakeDynabeadsTosylactivatedanexcellentchoiceforImmunoprecipitationofproteinsandproteincomplexes(Co-Immunoprecipitation,Co-IP).Gentleandrapidmagneticconcentrationofthebeadsandshortincubationtimes(possIBLeduetofastsurface-basedbindingkinetics)makeDynabeadsTosylactivatedanexcellentchoicefortheimmunoprecipitationofhighly-lABIleorand/ortransient(short-lived)proteincomplexes.
Ultrarapidprotocols:
•Identifymembersofproteincomplexesinminutesratherthanhours
•Temporalresolutionshortenoughtoidentifytransientandlabilecomplexes
•Identifybindingpartnersthatcannotbeidentifiedwithlongerprotocols
•Rapidprotocolsfurtherreducethealreadyultra-lowbackgroundbinding
•Strongersignaltonoise-ratios
Applications:
•IPofproteinsandproteincomplexes
•Couplefunctionalenzymestobeadsurfacefordownstreamassays
•Couplepeptidestobeadsurfacetoidentifybinders
Purifypeptides,proteinsandenzymesthatare:
•Transientlystable
•Structurallyintact
•Temperaturelabile
•Intheirnativeconformationandfunctional
CouplingProcedureOutline:
CovalentcouplingisperformedovernightbyincubatingthedesiredligandwiththeDynabeadsTosylactivated.LigandscommonlycoupledtoDynabeadsTosylactivatedincludepeptidesandproteins(e.g.antibodiesforImmunoprecipitationorCo-Immunoprecipitation).CouplingoccursatneutraltohighpHandat37°C.WerecommendcouplingatpH8.5-9.5,butforpHlabileligands,couplingcanbeperformedinanalternativebufferatpH7.4.
Uponcompletionoftheligandcouplingstep,theactualDynabeadsTosylactivatedsurfacecoatingwillberenderedinert,resultinginlownon-specificbinding.
AboutDynabeads:
Dynabeadsarenon-porousmonodispersesuperparamagneticbeads.Theyarehighlymobileinsolutionenablingligandscoupledtothebeadstocontinuouslyinteractwiththeentiresamplevolume.Thesuperparamagneticbeadsarepulledtothetubewallsbytransferringthetubetoarackcontainingastrongmagneticfield(FromtherednavigationbarnavigateBrands>Dynal®>Magnets).Strongmagneticfieldsquicklypullthebeadstothetubewall,allowingforeasyandcompleteremovalofthesupernatantbyPipette.Washingstepsareperformedsimilarly.l
BeadSurfaceCharacteristicsforDynabeadsTosylactivated:
•p-toluene-sulfonyl(Tosyl)groups
•Hydrophobic,pHNeutral
•Covalentbindingbyprimaryamine(NH2)orsulphydryl(SH)groups
Bindingcapacitypermilligrambeads:
Variesdependingonligand,(e.g.5-10µgIgG)
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
figure2.Magneticseparationtechnologyissimplewithveryfewhandlingsteps
Specifications
| SurfaceFunctionality: | Tosylactivated, p-Toluene-Sulfonyl |
|---|---|
| ElutionVolume-min: | 5µl |
| Form: | BeadsinSUSPension |
| HighThroughputCompatibility: | HighThroughput-Compatible |
| ProductLine: | DYNAL®, Dynabeads® |
| ProductSize: | 2mL |
| SampleType(Specific): | AnySampleType |
| ShelfLife: | 36monthsfromdateofmanufacture |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storethesebeadsat2-8°C.
品牌介绍
Pierce创立于1950年，现为生命科学和分析研究领域的领先者。在有机合成、化学偶联、表面化学、分析化学等方面具有强大实力，特色产品是优良的载体蛋白，交联试剂，蛋白及抗体的固化，非放射性标记，蛋白质分析及表达蛋白抽提试剂盒等，为单抗制备、纯化、修饰和蛋白质研究等方面的提供全新的解决方案，其化学发光技术也居于世界领先地位。
