Pierce/Dynabeads M-270 Epoxy/60 mg/14301
商品编号：
14301
品牌：
piercechemical
市场价：
¥7880.00
美元价：
4728.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
Dynabeads®M-270Epoxybeadsare2.8µmsuperparamagneticbeadscontainingsurfaceepoxygroups.Thebeadscovalentlybindprimaryaminoandsulfhydrylgroupsinproteinsandpeptides,makingthemidealforcouplingantibodies,peptides,intactproteins,andfunctionalenzymes.Thesehydrophylic,neutralpHbeadsexhibitextremelylownonspecificbindingofproteinsanddyes,whichreducestheneedforblockingagents.Also,Dynabeads®M-270EpoxyBeadsdonotcontainTween®detergent,andsoareidealforuseinmassspectrometry(MS)analysis.•Idealforimmunoprecipitation(IP)ofproteinsandproteincomplexes
•Gentle,yetrapidmagneticseparationandshortincubationtimesallowidentificationoftransientandlABIlecomplexes
•Covalentantibody-couplingtotheDynabeads®avoidsco-elutionoftheantibodywiththetargetprotein
Adaptableforvariousapplications
FastsurfacebindingkineticsandrapidmagneticseparationcharacteristicsmakeDynabeads®M-270Epoxybeadstheidealchoiceforapplicationssuchasimmunoprecipitation/purificationofproteinsandproteincomplexes,couplingfunctionalenzymestothebeadsurfacefordownstreamassays,andidentifyingproteinbindingpartners.Capturedproteinsandproteincomplexesareeasilyseparated,washed,andelutedusingaDynaMag™magnet,andthemagneticseparationpropertiesoftheDynabeads®.
Couplingprocedure
Covalentcouplingisperformedovernightbyincubatingthedesiredligand(commonlyantibodies,peptides,orproteins)withDynabeads®M-270Epoxybeads.Thebindingcapacityvariesdependingontheligandused,butistypically5–10µgIgGpermilligramofbeads.CouplingoccursatneutralpHandhighsaltconcentration.Thecouplingreactioncanbeperformedoverabroadrangeoftemperaturestomatchthestabilitycharacteristicsofyourligand.
Uponcompletionoftheligandcouplingstep,thereactivemoietiesontheDynabeads®M-270Epoxybeadsaredeactivatedtopreventfurthercoupling.Oncethecouplingreactioniscompleted,thesenonporous,hydrophilic,pHneutralbeadsexhibitextremelylownonspecificbinding.
LearnMoreaboutDynabeads®Products
•FindDynabeads®productsforawholerangeofapplications.
•FindmagnetsforDynabeads®separations.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
figure1.DirectcovalentbindingtoDynabeads®M-270Epoxy
Specifications
| SurfaceFunctionality: | Epoxy |
|---|---|
| ElutionVolume-min: | 5µl |
| Form: | FreezeDried |
| ProductLine: | DYNAL®, Dynabeads® |
| ProductSize: | 60mg |
| SampleType(Specific): | AnySampleType |
| ShelfLife: | 36monthsfromdateofmanufacture |
| ShippingCondition: | RoomTemperature |
Contents&storage
Contains60mgDynabeads®M-270Epoxy,~6.7×107beads/mg,suppliedasafreeze-driedpowder.
Storeat2°Cto8°C.
Storeat2°Cto8°C.
品牌介绍
Pierce创立于1950年，现为生命科学和分析研究领域的领先者。在有机合成、化学偶联、表面化学、分析化学等方面具有强大实力，特色产品是优良的载体蛋白，交联试剂，蛋白及抗体的固化，非放射性标记，蛋白质分析及表达蛋白抽提试剂盒等，为单抗制备、纯化、修饰和蛋白质研究等方面的提供全新的解决方案，其化学发光技术也居于世界领先地位。
