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Pierce/Qdot 655 Streptavidin Conjugate/200 µL/Q10121MP
Pierce/Qdot 655 Streptavidin Conjugate/200 µL/Q10121MP
商品编号：
Q10121MP
品牌：
piercechemical
市场价：
¥11080.00
美元价：
6648.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
Qdot®655streptavidinconjugatecomprisesabiotin-bindingprotein(streptavidin)covalentlyattachedtoafluorescentlabel(Qdot®nanocrystal).Streptavidinhasaveryhighbindingaffinityforbiotin,andaconjugateofstreptavidiniscommonlyusedtogetherwithaconjugateofbiotinforspecificdetectionofavarietyofproteins,proteinmotifs,nucleicacids,andothermolecules(forexample,abiotinylatedprimaryantibodyboundtoaproteintargetcanbedetectedwithafluorescentlylabeledstreptavidin).Strategiessimilartothisareusedinmanydetectionprotocolsincludingwesternblots,flowcytometry,imagingandmicroscopy,andmicroplateassays,andarealsousedinpurificationworkflowstoachievetargetedfractionation.Qdot®nanocrystalconjugatesareprovidedas1µMsolutions.ImportantFeaturesofQdot®StreptavidinConjugates:
•StreptavidinQdot®655conjugatehasemissionmaximaof~655nm
•Approximately5to10streptavidinsperQdot®nanocrystal
•Extremelyphotostableandbrightfluorescence
•Efficientlyexcitedwithsingle-lineexcitationsources
•Narrowemission,largeStokesshift
•Availableinmultiplecolors
•Idealforwesternblots,flowcytometry,imagingandmicroscopy,microplateassaysandmore
PropertiesofQdot®Nanocrystals
TheQdot®streptavidinconjugateisthesizeofalargemacromoleculeorprotein(~15–20nm)andrepresentsthebrightestclassofstreptavidindetectionreagents.Qdot®streptavidinconjugatesaremadefromananometer-scalecrystalofasemiconductormaterial(CdSe),whichiscoatedwithanadditionalsemiconductorshell(ZnS)toimprovetheopticalpropertiesofthematerial.TheQdot®705andQdot®800streptavidinconjugates,whichincludeCdSeTe,aremadeinasimilarfashion.Thiscore-shellmaterialisfurthercoatedwithapolymershellthatallowsthematerialstobeconjugatedtoBIOLOGicalmoleculesandtoretaintheiropticalproperties.
OtherFluorescentConjugatesofStreptavidinareAvailable
WeofferseveralotherQdot®colorsortrytheQdot®StreptavidinSamplerKit,containingQdot®streptavidinconjugatesinsixcolors525,565,585,605,655,and705.Inadditiontonanocrystalconjugates,weofferawiderangeofstreptavidinsconjugatedtoAlexaFluor®dyes,OregonGreen®dye,enzymeconjugates,andtrADItionalfluorophoreslikeTexasRed®dye,fluorescein(FITC)andmore.
FindBiotinylatedConjugates
Weofferanextensivelineofbiotinylatedconjugatesforuseinbiotin-streptavidindetectionstrategies.
•Usetheprimaryantibodysearchtooltofindbiotinylatedprimaryantibodies
•Usethesecondaryantibodyselectortooltofindbiotinylatedsecondaryantibodiesandbiotinylatedanti-dyeandanti-haptenantibodies
BlockingEndogenousBiotin
Naturallyoccurringbiotinscaninterferewithbiotin-streptavidindetectionschemes.ForexperimentsinvolvingfixedandpermeABIlizedcells,tryourEndogenousBiotin-BlockingKittominimizethisinterference.
ForResearchUseOnly.Notintendedforanyanimalorhumantherapeuticordiagnosticuse.
RelatedLinks:
LearnmoreaboutAvidin-BiotinDetection
TolearnmoreaboutQdot®Nanocrystals
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
FlowcytometryanalysisusingQDotnanocrystalsHMNClabeledwithabiotinylatedmouseanti-humanCD3antibodyfollowedbyastreptavidinconjugateofQdot655,werewashedandstainedwithabiotinylatedmouseanti-humanCD19antibodyfollowedbyaQdot605streptavdinconjugate.
Specifications
| LabelorDye: | Qdot®655 |
|---|---|
| ProductLine: | Qdot® |
| ProductSize: | 200µL |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storeinrefrigerator(2–8°C).
品牌介绍
Pierce创立于1950年，现为生命科学和分析研究领域的领先者。在有机合成、化学偶联、表面化学、分析化学等方面具有强大实力，特色产品是优良的载体蛋白，交联试剂，蛋白及抗体的固化，非放射性标记，蛋白质分析及表达蛋白抽提试剂盒等，为单抗制备、纯化、修饰和蛋白质研究等方面的提供全新的解决方案，其化学发光技术也居于世界领先地位。
