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Pierce/Amyloid beta 42 Mouse ELISA Kit/96 tests/KMB3441
Pierce/Amyloid beta 42 Mouse ELISA Kit/96 tests/KMB3441
商品编号：
KMB3441
品牌：
piercechemical
市场价：
¥12000.00
美元价：
7200.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
TheMouseBetaAmyloid1-42(Aβ42)ELISAresearchuseonlykitistobeusedforthequantitativedeterminationofMouseandRatAβ42insamples(e.g.,cellculturesupernatants,tissuehomogenates,cerebrospinalfluid[CSF],etc.)using96-wellplatesandamicroplatereader.ThisassaywasvalidatedusingcellculturesupernatantfromaNeuro-2acellculturewhichreleasesMouseAβ42intothesurroundingmedium.TheassaywillrecognizebothnaturalandsyntheticformsofMouseandRatAβ42.Theanti-mouseAβ42antibodyusedinthiskitiscapableofselectivelydetectingAβ42andnotAβ40orAβ43.*Performancecharacteristics
•Sensitivity:<3>
•Standardcurverange:3.12–200pg/mL
•Sampletypes:cellculturesupernatants,tissuehomogenates,cerebrospinalfluid(CSF)
•Speciescross-reactivity:mouse,rat
•Samplevolume:100μL(prediluted)
•Totalassayincubationtime:4hrs
Principleofthemethod
TheMouseAβ42kitisasolidphasesandwichEnzyme-LinkedImmunosorbentAssay(ELISA).AmonoclonalantibodytotheNH2-terminusofMouseAβhasbeencoatedontothewellsofthemicrotiterstripsprovided.Samples,includingstandardsofknownMouseAβ42content,controlspecimensandunknowns,arePipettedintothesewells.TheAβantigenbindstotheimmobilized(capture)antibody.Afterwashing,arabbitmonoclonalantibody*specificfortheCOOH-terminusofthe1-42Aβsequenceisaddedtothewells.BoundrabbitantibodyisdetectedbytheuseofahorserADIshperoxidase-labeledanti-rabbitantibody.
Afterremovalofexcessanti-rabbitantibody,asubstratesolutionisadded,whichisacteduponbytheboundenzymetoproducecolor.TheintensityofthiscoloredproductisdirectlyproportionaltotheconcentrationofMouseAβ42presentintheoriginalspecimen.
Backgroundinformation
Alzheimer’sDisease(AD)ischaracterizedbythepresenceofextracellularplaquesandintracellularneurofibrillarytangles(NFTs)inthebrain.Themajorproteincomponentoftheseplaquesisbetaamyloidpeptide(Aβ),a40to43aminoacidpeptidecleavedfromamyloidprecursorproteinbyβ-secretase(e.g.,BACE)andaputativeγ(gamma)secretase.Increasedreleaseofthe‘longerforms’ofAβpeptide,Aβ42orAβ43,whichhaveagreatertendencytoaggregatethanAβ40,occursinindividualsexpressingcertaingeneticmutations,expressingcertainApoEalleles,ormayinvolveother,stillundiscovered,factors.ManyresearcherstheorizethatitisthisincreasedreleaseofAβ42/Aβ43whichleadstotheabnormaldepositionofAβandtheassociatedneurotoxicityinthebrainsofaffectedindividuals.InpatientswithAD,reducedlevelsofAβ42inCSFhavebeendescribedaspredictiveofAD.
EachMouseAβ42ELISAkitisvalidatedforsensitivity,specificity,precision,andlot-to-lotconsistency.Seeproductinsertformoreinformationonvalidation.
Relatedlinks
LearnmoreaboutELISAkits
Learnmoreaboutotherimmunoassays
*PleaserefertotheELISAprotocolforspecificreferencecitations.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
Parallelism.
Specifications
| AssayIncubationTime: | 4hr |
|---|---|
| AssayRange: | 3.12-200pg/mL |
| AssaySensitivity: | <3pg/mL |
| DetectionMethod: | Colorimetric |
| ForUseWith(Equipment): | MicroplateReader |
| FormerIdentifiers: | Novex |
| GeneAliases: | AAA,ABETA,ABPP,AD1I,CTFgamma,CVAP,PN-II,PN2,APP |
| GeneID: | 351 |
| GeneName: | amyloidbetaprecursorprotein |
| GeneSymbol: | APP |
| ImmunoassayKitFormat: | CoatedELISAKit |
| LabelorDye: | HRP(HorseradishPeroxidase) |
| ProductSize: | 96tests |
| ProteinName: | AmyloidbetaA4protein |
| SampleType(Specific): | CellCultureSupernatants, CerebrospinalFluid, TissueHomogenates |
| SampleVolume: | 100µL |
| ShippingCondition: | WetIce |
| Target(KitNamed): | Amyloidbeta42 |
| TargetSpecies(Named): | Mouse |
| TargetSpecies(Validated): | Mouse |
| UniProtID: | P05067 |
Contents&storage
Pre-coatedplate(s),standardorcalibrator,detectorantibody,HRPconjugate,diluents,washbuffer,substrate(s),andstopsolution.Storekitat2-8°C.SeeproductmanualfordetailedcontentsandstorageconditionsformaximumstABIlity.
品牌介绍
Pierce创立于1950年，现为生命科学和分析研究领域的领先者。在有机合成、化学偶联、表面化学、分析化学等方面具有强大实力，特色产品是优良的载体蛋白，交联试剂，蛋白及抗体的固化，非放射性标记，蛋白质分析及表达蛋白抽提试剂盒等，为单抗制备、纯化、修饰和蛋白质研究等方面的提供全新的解决方案，其化学发光技术也居于世界领先地位。
