Pierce/c-Met（可溶性）人ELISA试剂盒/96试验/KHO2031
商品编号：
KHO2031
品牌：
piercechemical
市场价：
¥8980.00
美元价：
5388.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
TheHumanc-Met(Soluble)ELISAresearch-use-onlykitisdesignedtodetectandquantifythelevelofsolublehumanc-Metproteininserum,plasma,andcellculturesupernatantsusing96-wellplatesandamicroplatereader.Theassaywillrecognizebothnaturalandrecombinantsolublehumanc-Met.Performancecharacteristics
•Sensitivity:<0.5>
•Standardcurverange:0.78–50ng/mL
•Sampletypes:serum,plasma,cellculturesupernatants
•Speciescross-reactivity:human
•Samplevolume:100μL(1:2to1:100)
•Totalassayincubationtime:4hrs
Principleofthemethod
TheHumanc-Met(Soluble)kitisasolidphasesandwichenzyme-linkedimmunosorbentassay(ELISA).Ahighlypurifiedantibodyhasbeencoatedontothewellsofthemicrotiterstripsprovided.Duringthefirstincubation,standardsofknownsolublehumanc-Metcontent,controls,andunknownsamplesarePipettedintothewells.Afterwashing,biotinylatedsecondaryantibodyisadded.Afterwashing,streptavidin-peroxidase(enzyme)isadded.Thisbindstothebiotinylatedantibodytocompletethefour-membersandwich.Afterathirdincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionisadded,whichisacteduponbytheboundenzymetoproducecolor.Theintensityofthiscoloredproductisdirectlyproportionaltotheconcentrationofsolublehumanc-Metpresentintheoriginalspecimen.
Backgroundinformation
c-Met,amemberofthetyrosinekinasesuperfamily,isthereceptorforhepatocytegrowthfactor,alsoknownasscatterfactor(HGF/SF).Cellsexpressingc-Metincludeepithelialcells,endothelialcells,bloodcellsofvarioustypes,andglomerularmesenchymalcells.SourcesofHGF/SFincludemesenchymalcells,mesanglialcells,endothelialcells,macrophages,andtumorcells.
HGF/SFbindingtoc-Metstimulatesreceptordimerizationandthephosphorylationofnumerousresidueswithinthereceptor’scytoplasmicdomain.Phosphorylationoftyrosines1234and1235ofc-Metisrequiredforactivationofthereceptor’styrosinekinaseactivity.c-Metphosphorylationalsogeneratesdockingsitesfornumeroussignalingmoleculesandstimulatesreceptorinternalizationviaclathrin-coatedvesicles.Signalingproteinsthatarephosphorylatedand/orlocalizedinresponsetoc-Metphosphorylationinclude:Grb2,Shc,Cbl,Crk,cortactin,paxillin,GAB1,PI-3K,FAK,Src,Ras,ERK1and2,JNK,PLC-γ,AKT,andSTAT3.HGF/SFstimulationofc-Metexpressingcellsenhancesproliferation,migration,morphogenesis,andproteasesynthesis,characteristicsthatareassociatedwithinvasivecellphenotype.
Solublec-Metisatruncatedformofthec-Metmembranereceptor.Itcanbecleavedbyproteasesandreleasedfromthelipidbilayerinaprocessknownasectodomainshedding.Manytransmembraneproteinsarereleasedthroughthissheddingprocessanditisanormalpartofdevelopmentwhichwhendefectivecancauseanumberofpathologies.Thesolubleformofthec-Metreceptorissmallerthanthemembraneboundreceptor,containstheextracellularregionofthereceptor,andisabletobindtheHGFligand.
Detectlow-expressingproteinsanduselesssample
ELISAkitsdesignedtomeasureintracellularsignalingtargetsaretypically2–10timesmoresensitivethanwesternblotting.Theimprovedsensitivityenablesyoutodetectlow-expressingproteinsthatotherwisemaynotbedistinguishablefrombackground.Inaddition,theamountofsampleneededtoruntheassayislessthanwhatisneededforwesternblots.
EachELISAkitisvalidatedforsensitivity,specificity,precision,andlot-to-lotconsistency.Seeproductinsertformoreinformationonvalidation.
Relatedlinks
LearnmoreaboutELISAkits
Learnmoreaboutotherimmunoassays
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Specifications
| ProteinFamily: | Kinases&Inhibitors |
|---|---|
| AssayIncubationTime: | 4hr |
| AssayRange: | 0.78-50ng/mL |
| AssaySensitivity: | <0.5 ng/mL |
| DetectionMethod: | Colorimetric |
| ForUseWith(Equipment): | MicroplateReader |
| FormerIdentifiers: | Novex |
| GeneAliases: | AUTS9,DFNB97,HGFR,RCCP2,c-Met |
| GeneID: | 4233 |
| GeneName: | METproto-oncogene,receptortyrosinekinase |
| GeneSymbol: | MET |
| ImmunoassayKitFormat: | CoatedELISAKit |
| LabelorDye: | HRP(HorserADIshPeroxidase) |
| ProductSize: | 96tests |
| ProteinName: | Hepatocytegrowthfactorreceptor |
| ProteinSubtype: | ReceptorTyrosineKinases |
| SampleType(Specific): | CellCultureSupernatants, Plasma, Serum |
| SampleVolume: | 100µL |
| ShippingCondition: | WetIce |
| Target(KitNamed): | c-Met |
| TargetSpecies(Named): | Human |
| TargetSpecies(Validated): | Human |
| TargetSub-Specificity: | Soluble |
| UniProtID: | P08581 |
Contents&storage
Pre-coatedplate(s),standardorcalibrator,detectorantibody,HRPconjugate,diluents,washbuffer,substrate(s),andstopsolution.Storekitat2-8°C.SeeproductmanualfordetailedcontentsandstorageconditionsformaximumstABIlity.
品牌介绍
Pierce创立于1950年，现为生命科学和分析研究领域的领先者。在有机合成、化学偶联、表面化学、分析化学等方面具有强大实力，特色产品是优良的载体蛋白，交联试剂，蛋白及抗体的固化，非放射性标记，蛋白质分析及表达蛋白抽提试剂盒等，为单抗制备、纯化、修饰和蛋白质研究等方面的提供全新的解决方案，其化学发光技术也居于世界领先地位。
