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Pierce/ERK1/ERK2（磷酸）[pT185/pY187]人ELISA试剂盒/96试验/KHO0091
![Pierce/ERK1/ERK2（磷酸）[pT185/pY187]人ELISA试剂盒/96试验/KHO0091](https://www.ebiomall.cn/images/201711/1510882825526860127.jpg)
Pierce/ERK1/ERK2（磷酸）[pT185/pY187]人ELISA试剂盒/96试验/KHO0091
商品编号：
KHO0091
品牌：
piercechemical
市场价：
¥9980.00
美元价：
5988.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
TheERK1/2[pTpY185/187]ELISAresearchuseonlykitisdesignedtodetectandquantifythelevelofERK2proteinphosphorylatedatthreonineresidue185andtyrosine187andERK1atthreonine202andtyrosine204.ThisassayisintendedforthedetectionofERK1/2[pTpY185/187]fromhumancelllysatesusing96-wellplatesandamicroplatereader.•Easytorun
•Moresensitivethanwesterns
•Convenientprecoated,removable8-wellstrips
Easy-to-RunSandwichELISA
Thetotalassayincubationtimeisonly4hours.AmonoclonalcaptureantibodyspecificforERK1/2hasbeencoatedontothewellsofthe96-wellplateprovided.Duringthefirstincubation,standardsofknownERK1/2[pTpY185/187]contentandunknownsamplesarePipettedintothewellsandtheERK1/2antigenbindstotheimmobilized(capture)antibody.Afterwashing,arabbitantibodyspecificforERK1/2[pTpY185/187]isaddedtothewells.Duringthesecondincubation,thisantibodyservesasadetectionantibodybybindingtotheimmobilizedERK1/2proteincapturedduringthefirstincubation.Afterwashing,ahorserADIshperoxidase–labeledanti–rabbitIgGisadded.Thisbindstothedetectionantibodytocompletethefour–membersandwich.Afterathirdincubationandwashingtoremovealltheunboundenzyme,asubstratesolution(TMB)isadded,whichisacteduponbytheboundenzymetoproducecolor.TheintensityofthiscoloredproductisdirectlyproportionaltotheconcentrationofERK1/2[pTpY185/187]presentintheoriginalspecimenandtheopticaldensitycanbereadonastandardmicroplatereader.
DetectLow-ExpressingProteinsandUseLessSample
ELISAkitsdesignedtomeasureintracellularsignalingtargetsare2–10timesmoresensitivethanwesternblotting.Theimprovedsensitivityenablesyoutodetectlow-expressingproteinsthatotherwisemaynotbedistinguishablefrombackground.Inaddition,theamountofsampleneededtoruntheassayislessthanwhatisneededforwesternblots.
Ready-to-UseERK1/2[pTpY185/187]ELISAKitContainsTheseReagents,Buffers,andSupplies:
•ERK1/2AntibodyCoated96-WellPlate
•ERK1/2[pTpY185/187]Standard
•StandardDiluentBuffer
•ERK1/2[pTpY185/187]DetectionAntibody
•Anti-RabbitIgG-HRP(100X)
•HRPDiluent
•WashBufferConcentrate(25X)
•StABIlizedChromogen,TMB
•StopSolution
•PlateCovers
•Detailedprotocolwithvalidationtests
ConvenientKitFormat
Thekitcomeswithacaptureantibodyprecoatedtotheplate,eliminatinganovernightcoatingprocess.Theplatesaremachinecoatedtoprovidelowvariabilitywell-to-well.FurThermore,theplatecomesasremovable8-wellstripsallowingyoutorunasfewsamplesasyouwant.Inaddition,thestandardsprovidedinthekitcanbeusedtogetquantitativeresultsoffthestandardcurveand/orcanbeusedasapositivecontrol.
RigorousValidation
EachELISAkitisvalidatedforsensitivity,specificity,precision,andlot-to-lotconsistency.Seeprotocolinsertformoreinformationonvalidation.
PartoftheELISAKitFamily
WehaveawidemenuofELISAresearchuseonlykits.Withexcellentsensitivity,provenspecificity,andconsistentperformance,theseoptimizedELISAkitsofferyouachoicewhenitcomestogettingphysiologicallyrelevantandquantitativeresults.
Forresearchuseonly.Notintendedforhumanoranimaltherapeuticordiagnosticuse.
RelatedLinks:
LearnmoreaboutELISAKits
LearnmoreaboutotherImmunoassays
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
Parallelism.
ERKphosphoELISAâ„¢.
![ERK1&2 [pTpY185/187] phosphoELISAâ„¢ (Cat. No. KHO0091).](images/thermo/KHO0091_SC_080425.jpg-650.jpg)
ERK1&2[pTpY185/187]phosphoELISAâ„¢(Cat.No.KHO0091).
Specifications
| ProteinFamily: | Kinases&Inhibitors |
|---|---|
| AssayIncubationTime: | 4hr |
| AssayRange: | 1.6U/mL |
| AssaySensitivity: | <0.8Units/mL |
| DetectionMethod: | Colorimetric |
| ForUseWith(Equipment): | MicroplateReader |
| FormerIdentifiers: | Novex |
| GeneAliases: | ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK, ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK |
| GeneID: | 5594, 5595 |
| GeneName: | mitogen-activatedproteinkinase1, mitogen-activatedproteinkinase3 |
| GeneSymbol: | MAPK1, MAPK3 |
| ImmunoassayKitFormat: | CoatedELISAKit |
| LabelorDye: | HRP(HorseradishPeroxidase) |
| ProductSize: | 96tests |
| ProteinName: | Mitogen-activatedproteinkinase1, Mitogen-activatedproteinkinase3 |
| ProteinSubtype: | Serine⁄ThreonineKinases |
| SampleType(Specific): | CellExtracts |
| SampleVolume: | 100µL |
| ShippingCondition: | WetIce |
| Target(KitNamed): | ERK1/ERK2 |
| TargetSite-Specificity: | [pT185/pY187] |
| TargetSpecies(Named): | Human |
| TargetSpecies(Validated): | Human, Mouse, Rat |
| TargetSub-Specificity: | Phospho |
| UniProtID: | P27361, P28482 |
Contents&storage
Pre-coatedplate(s),standardorcalibrator,detectorantibody,HRPconjugate,diluents,washbuffer,substrate(s),andstopsolution.Storekitat2-8°C.Seeproductmanualfordetailedcontentsandstorageconditionsformaximumstability.
品牌介绍
Pierce创立于1950年，现为生命科学和分析研究领域的领先者。在有机合成、化学偶联、表面化学、分析化学等方面具有强大实力，特色产品是优良的载体蛋白，交联试剂，蛋白及抗体的固化，非放射性标记，蛋白质分析及表达蛋白抽提试剂盒等，为单抗制备、纯化、修饰和蛋白质研究等方面的提供全新的解决方案，其化学发光技术也居于世界领先地位。
